Microbiological diagnosis of bacteraemia and fungaemia: Blood cultures and molecular methods
Por:
Guna Serrano MR, Larrosa Escartín N, Marín Arriaza M and Rodríguez Díaz JC
Publicada:
1 may 2019
Ahead of Print:
21 abr 2018
Resumen:
Objetive: In this review we try to update the new procedures applicable in the microbiological diagnosis of bacteriemia and fungemias.
Method: Review of scientific literature.
Results and conclusions: After defining the process and indicating its fundamental principles, the main biomarkers used in clinical practice are reviewed. Subsequently, the particularities of the pre-analytical phase (collection and transport of samples) are highlighted and the steps to follow for the microbiological identification by classical methods are detailed, based on the culture of the blood samples. In the following section, we review the diagnostic methods not culture based, including those that detect the presence of the genome of the microorganism and those based on the study of proteome by mass spectrometry. The last section describes the procedures more frecuently used for the study of antibiotic susceptibility, both by phenotypic and genotypic methods. (C) 2018 Elsevier Espana, S.L.U. and Sociedad Espanola de Enfermedades Infecciosas y Microbiologia Clinica. All rights reserved.
Filiaciones:
Guna Serrano MR:
Servicio de Microbiología, Consorcio Hospital General Universitario de Valencia, Valencia, España
Larrosa Escartín N:
Servicio de Microbiología, Hospital Universitario Vall d'Hebron, Barcelona, España
Marín Arriaza M:
Servicio de Microbiología Clínica y Enfermedades Infecciosas, Hospital General Universitario Gregorio Marañón, Madrid, España
:
Servicio de Microbiología, Hospital General Universitario de Alicante, Instituto Sanitario y Biomédico de Alicante (ISABIAL), Alicante, España
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