Molecular Survey of Rickettsia spp., Anaplasma spp., Ehrlichia spp., Bartonella spp., and Borrelia spp. in Fleas and Lice in Ethiopia.
Por:
Perez-Tanoira, R, Ramos-Rincon, J, Martin-Martin, I, Prieto-Perez, L, Tefasmariam, A, Tiziano, G, Anda, P, Gonzalez-Martin-Nino, R, Rodriguez-Vargas, M, Gorgolas, M and Jado, I
Publicada:
1 ene 2020
Ahead of Print:
1 ago 2019
Resumen:
Bacterial arthropod-borne pathogens can often cause fever in Africa, but rural laboratories in these settings are usually too basic to provide a precise picture of their epidemiological impact. Our aim was to determine the prevalence of bacterial pathogens in fleas and lice in a rural area of southeast Ethiopia. Between July and November 2013, we extracted DNA from 91 fleas ( Ctenocephalides felis [ n = 50; 54.9%], Pulex irritans [ n = 37; 40.1%], and C. canis [ n = 4; 4.4%] and 30 lice ( Pediculus humanus capitis [ n = 16; 53.3%] and Pediculus humanus humanus [ n = 14; 46.7%]), using two quantitative PCR (qPCR) analyses to look for bacteria from the genera: Anaplasma , Bartonella , Borrelia , Coxiella , Ehrlichia , Francisella , and Rickettsia . Of the 91 fleas analyzed, pathogens were present in 79 (86.8%), including Rickettsia felis ( n = 41; 45%), Anaplasma platys ( n = 40; 44.0%), Rickettsia monacensis ( n = 2; 2.2%), Ehrlichia muris- like agent ( n = 1; 1.1%), and Bartonella clarridgeiae ( n = 1; 1.1%). P. irritans was the flea species most frequently infected with A. platys (67.7%), followed by C. felis (30.7%) ( p < 0.001). Of the 30 lice identified, pathogens were present in 7 (23.3%): Bartonella quintana ( n = 4; 16.7%), E. muris ( n = 2, 6.7%), and Borrelia recurrentis ( n = 1, 3.3%). Thus, in this rural area of Africa, fleas and lice can transmit parasitic pathogens to humans, causing febrile symptoms.
Filiaciones:
Perez-Tanoira, R:
1Department of Medicine and Laboratory, Gambo Rural General Hospital, Kore, Ethiopia
2Department of Infectious Diseases, IIS-Fundación Jiménez Díaz and Universidad Autónoma de Madrid, Madrid, Spain
:
1Department of Medicine and Laboratory, Gambo Rural General Hospital, Kore, Ethiopia
3Department of Internal Medicine, General University Hospital of Alicante, Institute for Health and Biomedical Research of Alicante (ISABIAL-Foundation FISABIO) and Miguel Hernández University of Elche, Alicante, Spain
Martin-Martin, I:
4National Centre of Microbiology, Health Institute Carlos III, Madrid, Spain
Prieto-Perez, L:
1Department of Medicine and Laboratory, Gambo Rural General Hospital, Kore, Ethiopia
2Department of Infectious Diseases, IIS-Fundación Jiménez Díaz and Universidad Autónoma de Madrid, Madrid, Spain
Tefasmariam, A:
1Department of Medicine and Laboratory, Gambo Rural General Hospital, Kore, Ethiopia
Tiziano, G:
1Department of Medicine and Laboratory, Gambo Rural General Hospital, Kore, Ethiopia
Anda, P:
4National Centre of Microbiology, Health Institute Carlos III, Madrid, Spain
Gonzalez-Martin-Nino, R:
4National Centre of Microbiology, Health Institute Carlos III, Madrid, Spain
Rodriguez-Vargas, M:
4National Centre of Microbiology, Health Institute Carlos III, Madrid, Spain
Gorgolas, M:
1Department of Medicine and Laboratory, Gambo Rural General Hospital, Kore, Ethiopia
2Department of Infectious Diseases, IIS-Fundación Jiménez Díaz and Universidad Autónoma de Madrid, Madrid, Spain
Jado, I:
4National Centre of Microbiology, Health Institute Carlos III, Madrid, Spain
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