Deciphering Pathways for Carotenogenesis in Haloarchaea
Por:
Giani, M, Miralles-Robledillo, J, Peiro, G, Pire, C and Martinez-Espinosa, R
Publicada:
1 mar 2020
Resumen:
Bacterioruberin and its derivatives have been described as the major carotenoids produced by haloarchaea (halophilic microbes belonging to the Archaea domain). Recently, different works have revealed that some haloarchaea synthetize other carotenoids at very low concentrations, like lycopene, lycopersene, cis- and trans-phytoene, cis- and trans-phytofluene, neo-beta -carotene, and neo-alpha -carotene. However, there is still controversy about the nature of the pathways for carotenogenesis in haloarchaea. During the last decade, the number of haloarchaeal genomes fully sequenced and assembled has increased significantly. Although some of these genomes are not fully annotated, and many others are drafts, this information provides a new approach to exploring the capability of haloarchaea to produce carotenoids. This work conducts a deeply bioinformatic analysis to establish a hypothetical metabolic map connecting all the potential pathways involved in carotenogenesis in haloarchaea. Special interest has been focused on the synthesis of bacterioruberin in members of the Haloferax genus. The main finding is that in almost all the genus analyzed, a functioning alternative mevalonic acid (MVA) pathway provides isopentenyl pyrophosphate (IPP) in haloarchaea. Then, the main branch to synthesized carotenoids proceeds up to lycopene from which beta -carotene or bacterioruberin (and its precursors: monoanhydrobacterioriberin, bisanhydrobacterioruberin, dihydrobisanhydrobacteriuberin, isopentenyldehydrorhodopsin, and dihydroisopenthenyldehydrorhodopsin) can be made.
Filiaciones:
Giani, M:
Univ Alicante, Fac Sci, Agrochem & Biochem Dept, Biochem & Mol Biol Div, Ap 99, Alicante 03690, Spain
Miralles-Robledillo, J:
Univ Alicante, Fac Sci, Agrochem & Biochem Dept, Biochem & Mol Biol Div, Ap 99, Alicante 03690, Spain
:
Univ Gen Hosp Alicante, Pathol Dept, Pintor Baeza 12, Alicante 03010, Spain
Univ Gen Hosp Alicante, Res Unit, Pintor Baeza 12, Alicante 03010, Spain
Pire, C:
Univ Alicante, Fac Sci, Agrochem & Biochem Dept, Biochem & Mol Biol Div, Ap 99, Alicante 03690, Spain
Martinez-Espinosa, R:
Univ Alicante, Fac Sci, Agrochem & Biochem Dept, Biochem & Mol Biol Div, Ap 99, Alicante 03690, Spain
gold, Green Published
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