Assessment of a subset of Slowly Mutating Y-STRs for forensic and evolutionary studies.
Por:
Baeta M, Núñez C, Villaescusa P, Ortueta U, Ibarbia N, Herrera RJ, Blazquez-Caeiro JL, Builes JJ, Jiménez-Moreno S, Martínez-Jarreta B and de Pancorbo MM
Publicada:
1 may 2018
Ahead of Print:
20 mar 2018
Resumen:
Y-specific short tandem repeat (Y-STR) loci display different mutation rates and consequently are suitable for forensic, genealogical, and evolutionary studies that require different levels of timelines and resolution. Recent efforts have focused on implementing Rapidly Mutating (RM) Y-STRs to assess male specific profiles. However, due to their high mutation rate their use in kinship testing or in phylogenetic studies may be less reliable. In the present study, a novel Slowly Mutating Y-STR (SM) panel, including DYS388, DYS426, DYS461 (Y-GATA-A7.2), DYS485, DYS525, and DYS561, has been developed and evaluated in a sample set of 628 unrelated males from different worldwide populations. This panel is reproducible, sensitive, and robust for forensic applications and may be useful in conjunction with the common multiplexes, particularly in exclusion of kinship cases where minimal discrimination is reported employing the rapidly mutating Y-STR systems. Furthermore, SM Y-STR data may be of value in evolutionary studies to optimize the resolution of phylogenetic relationships generated with current Y-STR panel sets. In this study, we provide an extensive Y-STR allele and haplotype reference dataset for future applications.
Filiaciones:
Baeta M:
BIOMICs Research Group, Lascaray Research Center, University of the Basque Country UPV/EHU, Avda. Miguel de Unamuno, 3, 01006, Vitoria-Gasteiz, Spain
Núñez C:
BIOMICs Research Group, Lascaray Research Center, University of the Basque Country UPV/EHU, Avda. Miguel de Unamuno, 3, 01006, Vitoria-Gasteiz, Spain
Villaescusa P:
BIOMICs Research Group, Lascaray Research Center, University of the Basque Country UPV/EHU, Avda. Miguel de Unamuno, 3, 01006, Vitoria-Gasteiz, Spain
Ortueta U:
BIOMICs Research Group, Lascaray Research Center, University of the Basque Country UPV/EHU, Avda. Miguel de Unamuno, 3, 01006, Vitoria-Gasteiz, Spain
Ibarbia N:
BIOMICs Research Group, Lascaray Research Center, University of the Basque Country UPV/EHU, Avda. Miguel de Unamuno, 3, 01006, Vitoria-Gasteiz, Spain
Herrera RJ:
Department of Molecular Biology, Colorado College, Colorado Springs, CO, 80903, USA
Blazquez-Caeiro JL:
Departament of Zoology and Physical Anthropology, University of Santiago de Compostela, Spain
Builes JJ:
Genes SAS Laboratory, Medellín, Colombia
Institute of Biology, University of Antioquia, Medellín, Colombia
:
Área Medicina Legal y Forense, Departments of Patología y Cirugía, Universidad Miguel Hernández de Elche, Elche, Alicante, Spain
Martínez-Jarreta B:
Department of Forensic Medicine, University of Zaragoza, Zaragoza, Spain
de Pancorbo MM:
BIOMICs Research Group, Lascaray Research Center, University of the Basque Country UPV/EHU, Avda. Miguel de Unamuno, 3, 01006, Vitoria-Gasteiz, Spain. Electronic address:
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