A Hypoallergenic Polygalacturonase Isoform from Olive Pollen Is Implicated in Pollen-Pollen Cross-Reactivity.
Por:
Oeo-Santos C, Mas S, Quiralte J, Colás C, Blanca M, Fernández J, Feo Brito F, Villalba M and Barderas R
Publicada:
31 ago 2018
Ahead of Print:
31 ago 2018
Resumen:
BACKGROUND: Cross-reactivity reactions between allergenic polygalacturonases (PGs) from different biological sources, especially foods and pollens from the Oleaceae family, have been described using Salsola kali PG (Sal k 6). No PG from olive pollen has been characterized to date, hampering further knowledge about cross-reactions through PGs. OBJECTIVES: The aim of this work was to determine the potential allergenicity of the PG from olive pollen and clarify its role in cross-reactivity. METHODS: A cDNA-encoding olive pollen PG sequence was subcloned into the pET41b vector and used to transform BL21(DE3) Escherichia coli cells to produce a His-tag fusion recombinant protein. The allergenic properties of olive pollen PG were determined by immunoblotting and ELISA in comparison to Sal k 6. The cross-reactivity potential of the protein with other pollen sources was analyzed by inhibition immunoassays. RESULTS: The existence of other isoforms of Ole e 14 with different allergenicity was confirmed by proteomics and a meta-analysis of the recently reported olive genome. Sal k 6 showed a higher IgE recognition than Ole e 14 regardless of patient sensitization, suggesting the existence of more allergenic Ole e 14 isoforms in olive pollen. IgG and IgE inhibition assays supported the existence of cross-reactions between them and with other PGs from Oleaceae and Poaceae plant families. CONCLUSIONS: A new allergen from olive pollen, Ole e 14, has been identified, produced as a recombinant isoform, and structurally and immunologically characterized. Its role in cross-reactivity has been confirmed and, due to its smaller IgE binding capacity, it could have an important role for therapeutic purposes.
Filiaciones:
Oeo-Santos C:
Departamento Bioquímica y Biología Molecular I, Universidad Complutense de Madrid, Madrid, Spain
Mas S:
Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico, Universitat Politècnica de València, Valencia, Spain
Quiralte J:
Departamento Alergología, Hospital Universitario Virgen del Rocío, Sevilla, Spain
Colás C:
Hospital Clínico Universitario "Lozano Blesa", Zaragoza, Spain
Blanca M:
Unidad de Alergología, Hospital Universitario Regional de Málaga-IBIMA, UMA, Málaga, Spain
:
Departamento Medicina Clínica, Universidad Miguel Hernández, Alicante, Spain
Feo Brito F:
Allergy Section, General Hospital, Ciudad Real, Spain
Villalba M:
Departamento Bioquímica y Biología Molecular I, Universidad Complutense de Madrid, Madrid,
Barderas R:
Departamento Bioquímica y Biología Molecular I, Universidad Complutense de Madrid, Madrid, Spain
UFIEC, CROSADIS, ISCIII, Majadahonda, Spain
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