17 to 23: A novel complementary mini Y-STR panel to extend the Y-STR databases from 17 to 23 markers for forensic purposes.
Por:
Núñez C, Baeta M, Ibarbia N, Ortueta U, Jiménez-Moreno S, Blazquez-Caeiro JL, Builes JJ, Herrera RJ, Martínez-Jarreta B and de Pancorbo MM
Publicada:
1 abr 2017
Ahead of Print:
13 ene 2017
Resumen:
A Y-STR multiplex system has been developed with the purpose of complementing the widely used 17 Y-STR haplotyping (AmpFlSTR Y Filer® PCR Amplification kit) routinely employed in forensic and population genetic studies. This new multiplex system includes six additional STR loci (DYS576, DYS481, DYS549, DYS533, DYS570, and DYS643) to reach the 23 Y-STR of the PowerPlex® Y23 System. In addition, this kit includes the DYS456 and DYS385 loci for traceability purposes. Male samples from 625 individuals from ten worldwide populations were genotyped, including three sample sets from populations previously published with the 17 Y-STR system to expand their current data. Validation studies demonstrated good performance of the panel set in terms of concordance, sensitivity, and stability in the presence of inhibitors and artificially degraded DNA. The results obtained for haplotype diversity and discrimination capacity with this multiplex system were considerably high, providing further evidences of the suitability of this novel Y-STR system for forensic purposes. Thus, the use of this multiplex for samples previously genotyped with 17 Y-STRs will be an efficient and low-cost alternative to complete the set of 23 Y-STRs and improve allele databases for population and forensic purposes.
Filiaciones:
Núñez C:
BIOMICs Research Group, Lascaray Research Center, University of the Basque Country UPV/EHU. Avda. Miguel de Unamuno, Vitoria-Gasteiz, Spain
Baeta M:
BIOMICs Research Group, Lascaray Research Center, University of the Basque Country UPV/EHU. Avda. Miguel de Unamuno, Vitoria-Gasteiz, Spain
Ibarbia N:
BIOMICs Research Group, Lascaray Research Center, University of the Basque Country UPV/EHU. Avda. Miguel de Unamuno, Vitoria-Gasteiz, Spain
Ortueta U:
BIOMICs Research Group, Lascaray Research Center, University of the Basque Country UPV/EHU. Avda. Miguel de Unamuno, Vitoria-Gasteiz, Spain
:
Área Medicina Legal y Forense, Department of Patología y Cirugía, Universidad Miguel Hernández de Elche, Elche, Alicante, Spain
Blazquez-Caeiro JL:
Departamento de Zoología y Antropología Física, Universidad de Santiago de Compostela, Spain
Builes JJ:
Laboratorios Genes Ltd., Medellín, Colombia
Instituto de Biología, Universidad de Antioquia, Medellín, Colombia
Herrera RJ:
Department of Molecular Biology, Colorado College, Colorado Springs, CO, USA
Martínez-Jarreta B:
Department of Forensic Medicine, University of Zaragoza, Zaragoza, Spain
de Pancorbo MM:
BIOMICs Research Group, Lascaray Research Center, University of the Basque Country UPV/EHU. Avda. Miguel de Unamuno, Vitoria-Gasteiz, Spain
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