First national survey of the diagnosis of Helicobacter pylori infection in Clinical Microbiology Laboratories in Spain
Por:
Miqueleiz-Zapatero, A, Alba-Rubio, C, Domingo-Garcia, D, Canton, R, de la Pedrosa, E, Aznar-Cano, E, Leiva, J, Montes, M, Sanchez-Romero, I, Rodriguez-Diaz, J, Alarcon-Cavero, T and Grp Trabajo Diagnost Microbiol Hel
Publicada:
1 nov 2020
Ahead of Print:
25 ene 2020
Resumen:
Introduction: The aim of this study was to know, through a national survey, the methods and techniques used for the diagnosis of Helicobacter pylori (Hp) in the different Clinical Microbiology Services/Laboratories in Spain, as well as antibiotic resistance data.
Methods: The survey requested information about the diagnostic methods performed for Hp detection in Clinical Microbiology laboratories, including serology, stool antigen, culture from gastric biopsies, and PCR. In addition, the performance of antibiotic susceptibility was collected. Data on the number of samples processed in 2016, positivity of each technique and resistance data were requested. The survey was sent by email (October-December 2017) to the heads of 198 Clinical Microbiology Laboratories in Spain.
Results: Overall, 51 centers from 29 regions answered the survey and 48/51 provided Hp microbiological diagnostic testing. Concerning the microbiological methods used to diagnose Hp infection, the culture of gastric biopsies was the most frequent (37/48), followed by stool antigen detection (35/48), serology (19/48) and biopsy PCR (5/48). Regarding antibiotic resistance, high resistance rates were observed, especially in metronidazole and clarithromycin (over 33%).
Conclusion: Culture of gastric biopsies was the most frequent method for detection of Hp, but the immunochromatographic stool antigen test was the one with which the largest number of samples were analyzed. Nowadays, in Spain, it concerns the problem of increased antibiotic resistance to 'first-line' antibiotics. (C) 2019 Elsevier Espana, S.L.U. and Sociedad Espanola de Enfermedades Infecciosas y Microbiologia Clinica. All rights reserved.
Filiaciones:
Miqueleiz-Zapatero, A:
Hosp Univ La Princesa, Serv Microbiol, Madrid, Spain
Inst Invest Princesa IP, Madrid, Spain
Complejo Hosp Navarra, Serv Microbiol, Pamplona, Spain
Alba-Rubio, C:
Hosp Univ La Princesa, Serv Microbiol, Madrid, Spain
Inst Invest Princesa IP, Madrid, Spain
Univ Complutense Madrid, Fac Vet, Madrid, Spain
Domingo-Garcia, D:
Hosp Univ La Princesa, Serv Microbiol, Madrid, Spain
Inst Invest Princesa IP, Madrid, Spain
Canton, R:
Hosp Univ Ramon Y Cajal, Serv Microbiol, Madrid, Spain
Inst Ramon Y Cajal Invest Sanitaria IRYCIS, Madrid, Spain
de la Pedrosa, E:
Hosp Univ Ramon Y Cajal, Serv Microbiol, Madrid, Spain
Inst Ramon Y Cajal Invest Sanitaria IRYCIS, Madrid, Spain
Aznar-Cano, E:
Lab Clin Cent Madrid BR Salud, Madrid, Spain
Leiva, J:
Clin Univ Navarra, Serv Microbiol, Pamplona, Spain
Inst Invest Sanitaria Navarra IDISNA, Pamplona, Spain
Montes, M:
Hosp Univ Donostia, Serv Microbiol, Inst Biodonostia, San Sebastian, Spain
Sanchez-Romero, I:
Hosp Univ Puerta del Hierro, Serv Microbiol, Majadahonda, Spain
:
Hosp Gen Univ Alicante, Serv Microbiol, Alicante, Spain
Alarcon-Cavero, T:
Hosp Univ La Princesa, Serv Microbiol, Madrid, Spain
Inst Invest Princesa IP, Madrid, Spain
Open Access
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