Cationic Dendrimer G2-S16 Inhibits Herpes Simplex Type 2 Infection and Protects Mice Vaginal Microbiome.
Por:
Guerrero-Beltrán C, Garcia-Heredia I, Ceña-Diez R, Rodriguez-Izquierdo I, Serramía MJ, Martinez-Hernandez F, Lluesma-Gomez M, Martinez-Garcia M and Muñoz-Fernández MÁ
Publicada:
4 jun 2020
Ahead of Print:
4 jun 2020
Resumen:
The G2-S16 polyanionic carbosilane dendrimer is a promising microbicide that inhibits HSV-2 infection in vitro and in vivo in mice models. This G2-S16 dendrimer inhibits HSV-2 infection even in the presence of semen. Murine models, such as BALB/c female mice, are generally used to characterize host-pathogen interactions within the vaginal tract. However, the composition of endogenous vaginal flora remains largely undefined with modern microbiome analyses. It is important to note that the G2-S16 dendrimer does not change healthy mouse vaginal microbiome where Pseudomonas (10.2-79.1%) and Janthinobacterium (0.7-13%) are the more abundant genera. The HSV-2 vaginally infected female mice showed a significant microbiome alteration because an increase of Staphylococcus (up to 98.8%) and Escherichia (30.76%) levels were observed becoming these bacteria the predominant genera. BALB/c female mice vaginally-treated with the G2-S16 dendrimer and infected with the HSV-2 maintained a healthy vaginal microbiome similar to uninfected female mice. Summarizing, the G2-S16 polyanionic carbosilane dendrimer inhibits the HSV-2 infection in the presence of semen and prevents the alteration of mice female vaginal microbiome.
Filiaciones:
Guerrero-Beltrán C:
Immunology Section, Head Inmuno-Biology Molecular Laboratoy, Hospital General Universitario Gregorio Marañón, Instituto de Investigación Sanitaria Gregorio Marañón (IiSGM), Spanish HIV HGM BioBank, C/Dr. Esquerdo 46, 28007 Madrid, Spain
:
Department of Physiology, Genetics, and Microbiology, University of Alicante, C/San Vicente s/n, 03080 Alicante, Spain
Ceña-Diez R:
Immunology Section, Head Inmuno-Biology Molecular Laboratoy, Hospital General Universitario Gregorio Marañón, Instituto de Investigación Sanitaria Gregorio Marañón (IiSGM), Spanish HIV HGM BioBank, C/Dr. Esquerdo 46, 28007 Madrid, Spain
Rodriguez-Izquierdo I:
Immunology Section, Head Inmuno-Biology Molecular Laboratoy, Hospital General Universitario Gregorio Marañón, Instituto de Investigación Sanitaria Gregorio Marañón (IiSGM), Spanish HIV HGM BioBank, C/Dr. Esquerdo 46, 28007 Madrid, Spain
Serramía MJ:
Immunology Section, Head Inmuno-Biology Molecular Laboratoy, Hospital General Universitario Gregorio Marañón, Instituto de Investigación Sanitaria Gregorio Marañón (IiSGM), Spanish HIV HGM BioBank, C/Dr. Esquerdo 46, 28007 Madrid, Spain
Networking Research Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), 28029 Madrid, Spain
Martinez-Hernandez F:
Department of Physiology, Genetics, and Microbiology, University of Alicante, C/San Vicente s/n, 03080 Alicante, Spain
Lluesma-Gomez M:
Department of Physiology, Genetics, and Microbiology, University of Alicante, C/San Vicente s/n, 03080 Alicante, Spain
Martinez-Garcia M:
Department of Physiology, Genetics, and Microbiology, University of Alicante, C/San Vicente s/n, 03080 Alicante, Spain
Muñoz-Fernández MÁ:
Immunology Section, Head Inmuno-Biology Molecular Laboratoy, Hospital General Universitario Gregorio Marañón, Instituto de Investigación Sanitaria Gregorio Marañón (IiSGM), Spanish HIV HGM BioBank, C/Dr. Esquerdo 46, 28007 Madrid, Spain
Networking Research Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), 28029 Madrid, Spain
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