Flow cytometry detection of sustained humoral immune response (IgG plus IgA) against native spike glycoprotein in asymptomatic/mild SARS-CoV-2 infection
Por:
Pinero, P, Marco De la Calle, F, Horndler, L, Alarcon, B, Uribe Barrientos, M, Sarmiento, H and Tarin, F
Publicada:
21 may 2021
Ahead of Print:
21 may 2021
Resumen:
SARS-CoV-2 is the virus that causes the disease called COVID-19, which
has caused the worst pandemic of the century. Both, to know the
immunological status of general population and to evaluate the efficacy
of the vaccination process that is taking place around the world,
serological tests represent a key tool. Classic serological tests, based
on colorimetric techniques, such as ELISA or CLIA, continue to be the
most widely used option. However, a real improvement in results is still
needed. We developed a highly sensitive and specific FCM assay that
allows the detection of IgG and IgA antibodies, directed against the
native and functional S-protein of SARS-CoV-2 exposed on the membrane of
a transfected cell line, up to 8 months after infection.
Green Published, gold
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