Measurable residual disease in elderly acute myeloid leukemia: results from the PETHEMA-FLUGAZA phase 3 clinical trial


Por: Simoes, C, Paiva, B, Martinez-Cuadron, D, Bergua, J, Vives, S, Algarra, L, Tormo, M, Martinez, P, Serrano, J, Herrera, P, Ramos, F, Salamero, O, Lavilla, E, Gil, C, Lopez, J, Vidriales, M, Labrador, J, Falantes, J, Sayas, M, Ayala, R, Martinez-Lopez, J, Villar, S, Calasanz, M, Prosper, F, San-Miguel, J, Sanz, M, Montesinos, P and Programa Estudio Terapeutica Hemop

Publicada: 9 feb 2021
Resumen:
The value of measurable residual disease (MRD) in elderly patients with acute myeloid leukemia (AML) is inconsistent between those treated with intensive vs hypomethylating drugs, and unknown after semi-intensive therapy. We investigated the role of MRD in refining complete remission (CR) and treatment duration in the phase 3 FLUGAZA clinical trial, which randomized 283 elderly AML patients to induction and consolidation with fludarabine plus cytarabine (FLUGA) vs 5-azacitidine. After consolidation, patients continued treatment if MRD was >= 0.01% or stopped if MRD was <0.01%, as assessed by multidimensional flow cytometry (MFC). On multivariate analysis including genetic risk and treatment arm, MRD status in patients achieving CR (N - 72) was the only independent prognostic factor for relapse-free survival (RFS) (HR, 3.45; P = .002). Achieving undetectable MRD significantly improved RFS of patients with adverse genetics (HR, 0.32; P = .013). Longer overall survival was observed in patients with undetectable MRD after induction though not after consolidation. Although leukemic cells from most patients displayed phenotypic aberrancies vs their normal counterpart (N = 259 of 265), CD34 progenitors from cases with undetectable MRD by MFC carried extensive genetic abnormalities identified by whole-exome sequencing. Interestingly, the number of genetic alterations significantly increased from diagnosis to MRD stages in patients treated with FLUGA vs 5-azacitidine (2.2-fold vs 1.1-fold; P = .001). This study supports MRD assessment to refine CR after semi-intensive therapy or hypomethylating agents, but unveils that improved sensitivity is warranted to individualize treatment and prolong survival of elderly AML patients achieving undetectable MRD.

Filiaciones:
Simoes, C:
 Clin Univ Navarra, Dept Hematol, Pamplona, Spain

 Ctr Invest Med Aplicada CIMA, Pamplona, Spain

 Inst Invest Sanitaria Navarra IDISNA, Pamplona, Spain

Paiva, B:
 Clin Univ Navarra, Dept Hematol, Pamplona, Spain

 Ctr Invest Med Aplicada CIMA, Pamplona, Spain

 Inst Invest Sanitaria Navarra IDISNA, Pamplona, Spain

Martinez-Cuadron, D:
 Hosp Univ & Politecn La Fe, Valencia, Spain

Bergua, J:
 Hosp San Pedro de Alcantara, Caceres, Spain

Vives, S:
 Hosp Badalona Germans Trias & Pujol, Inst Catalan Oncol ICO, Badalona, Spain

Algarra, L:
 Hosp Gen Albacete, Albacete, Spain

Tormo, M:
 Hosp Clin Univ Valencia, Valencia, Spain

Martinez, P:
 Hosp Univ 12 Octubre, Madrid, Spain

Serrano, J:
 Hosp Reina Sofia, Cordoba, Spain

 Inst Maimonides Invest Biomed Cordoba IMIBIC, Cordoba, Spain

Herrera, P:
 Hosp Univ Ramon y Cajal, Madrid, Spain

Ramos, F:
 Hosp Univ Leon, Leon, Spain

Salamero, O:
 Hosp Univ Vall dHebron, Barcelona, Spain

Lavilla, E:
 Hosp Lucus Augusti, Lugo, Spain

:
 Hosp Gen Univ Alicante, Alicante, Spain

Lopez, J:
 Fdn Jimenez Diaz, Madrid, Spain

Vidriales, M:
 Hosp Clin Univ Salamanca, Salamanca, Spain

Labrador, J:
 Hosp Univ Burgos, Burgos, Spain

Falantes, J:
 Complejo Hosp Virgen del Rocio, Seville, Spain

Sayas, M:
 Univ Hosp Dr Peset, Valencia, Spain

Ayala, R:
 Hosp Univ 12 Octubre, Madrid, Spain

Martinez-Lopez, J:
 Hosp Univ 12 Octubre, Inst Invest Octubre 12, Madrid, Spain

 Univ Complutense, Madrid, Spain

 Ctr Nacl Invest Oncol CNIO, Madrid, Spain

Villar, S:
 Clin Univ Navarra, Dept Hematol, Pamplona, Spain

 Ctr Invest Med Aplicada CIMA, Pamplona, Spain

Calasanz, M:
 Ctr Invest Med Aplicada CIMA, Pamplona, Spain

 Univ Navarra, Dept Biochem, Navarra, Spain

Prosper, F:
 Clin Univ Navarra, Dept Hematol, Pamplona, Spain

 Ctr Invest Med Aplicada CIMA, Pamplona, Spain

San-Miguel, J:
 Clin Univ Navarra, Dept Hematol, Pamplona, Spain

 Ctr Invest Med Aplicada CIMA, Pamplona, Spain

 Inst Invest Sanitaria Navarra IDISNA, Pamplona, Spain

 Inst Salud Carlos III, Ctr Invest Biomed Red Canc CIBER ONC, Area Oncol, Madrid, Spain

Sanz, M:
 Hosp Univ & Politecn La Fe, Valencia, Spain

Montesinos, P:
 Hosp Univ & Politecn La Fe, Valencia, Spain
ISSN: 24739529





Blood Advances
Editorial
ELSEVIER, RADARWEG 29, 1043 NX AMSTERDAM, NETHERLANDS, Países Bajos
Tipo de documento: Article
Volumen: 5 Número: 3
Páginas: 760-770
WOS Id: 000617538500014
ID de PubMed: 33560390
imagen Green Published, gold

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