Role of a cryptic tRNA gene operon in survival under translational stress
Por:
Santamaria-Gomez, J, Rubio, M, Lopez-Igual, R, Romero-Losada, A, Delgado-Chaves, F, Bru-Martinez, R, Romero-Campero, F, Herrero, A, Ibba, M, de Alda, J and Luque, I
Publicada:
7 sep 2021
Resumen:
As compared to eukaryotes, bacteria have a reduced tRNA gene set encoding between 30 and 220 tRNAs. Although in most bacterial phyla tRNA genes are dispersed in the genome, many species from distinct phyla also show genes forming arrays. Here, we show that two types of arrays with distinct evolutionary origins exist. This work focuses on long tRNA gene arrays (L-arrays) that encompass up to 43 genes, which disseminate by horizontal gene transfer and contribute supernumerary tRNA genes to the host. Although in the few cases previously studied these arrays were reported to be poorly transcribed, here we show that the L-array of the model cyanobacterium Anabaena sp. PCC 7120, encoding 23 functional tRNAs, is largely induced upon impairment of the translation machinery. The cellular response to this challenge involves a global reprogramming of the transcriptome in two phases. tRNAs encoded in the array are induced in the second phase of the response, directly contributing to cell survival. Results presented here show that in some bacteria the tRNA gene set may be partitioned between a housekeeping subset, which constantly sustains translation, and an inducible subset that is generally silent but can provide functionality under particular conditions.
Filiaciones:
Santamaria-Gomez, J:
CSIC, Inst Bioquim Vegetal & Fotosintesis, E-41092 Seville, Spain
Univ Seville, E-41092 Seville, Spain
Rubio, M:
CSIC, Inst Bioquim Vegetal & Fotosintesis, E-41092 Seville, Spain
Univ Seville, E-41092 Seville, Spain
Ohio State Univ, Ctr RNA Biol, 484 West 12th Ave, Columbus, OH 43210 USA
Ohio State Univ, Dept Microbiol, 318 West 12th Ave, Columbus, OH 43210 USA
Lopez-Igual, R:
CSIC, Inst Bioquim Vegetal & Fotosintesis, E-41092 Seville, Spain
Univ Seville, E-41092 Seville, Spain
Romero-Losada, A:
CSIC, Inst Bioquim Vegetal & Fotosintesis, E-41092 Seville, Spain
Univ Seville, E-41092 Seville, Spain
Univ Seville, Dept Comp Sci & Artificial Intelligence, E-41012 Seville, Spain
Delgado-Chaves, F:
CSIC, Inst Bioquim Vegetal & Fotosintesis, E-41092 Seville, Spain
Univ Seville, E-41092 Seville, Spain
:
Univ Alicante, Fac Sci, Dept Agrochem & Biochem, E-03690 Alicante, Spain
Romero-Campero, F:
CSIC, Inst Bioquim Vegetal & Fotosintesis, E-41092 Seville, Spain
Univ Seville, E-41092 Seville, Spain
Univ Seville, Dept Comp Sci & Artificial Intelligence, E-41012 Seville, Spain
Herrero, A:
CSIC, Inst Bioquim Vegetal & Fotosintesis, E-41092 Seville, Spain
Univ Seville, E-41092 Seville, Spain
Ibba, M:
Ohio State Univ, Ctr RNA Biol, 484 West 12th Ave, Columbus, OH 43210 USA
Ohio State Univ, Dept Microbiol, 318 West 12th Ave, Columbus, OH 43210 USA
Chapman Univ, Schmid Coll Sci & Technol, One Univ Dr, Orange, CA 92866 USA
de Alda, J:
Univ Extremadura, Fac Formac Profesorado, Didact Ciencias Expt, E-10003 Caceres, Spain
Luque, I:
CSIC, Inst Bioquim Vegetal & Fotosintesis, E-41092 Seville, Spain
Univ Seville, E-41092 Seville, Spain
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