Differentiation of human adult-derived stem cells towards a neural lineage involves a dedifferentiation event prior to differentiation to neural phenotypes
Por:
Bueno, C, Martinez-Morga, M, Garcia-Bernal, D, Moraleda, J and Martinez, S
Publicada:
8 jun 2021
Resumen:
Although it has been reported that mesenchymal stem cells isolated from adult tissues can be induced to overcome their mesenchymal fate and transdifferentiate into neural cells, the findings and their interpretation have been challenged. The main argument against this process is that the cells rapidly adopt neuron-like morphologies through retraction of the cytoplasm rather than active neurite extension. In this study, we examined the sequence of biological events during neural differentiation of human periodontal ligament-derived stem cells (hPDLSCs), human bone marrow-derived stem cells (hBMSCs) and human dental pulp-derived stem cells (hDPSCs) by time-lapse microscopy. We have demonstrated that hPDLSCs, hBMSCs and hDPSCs can directly differentiate into neuron-like cells without passing through a mitotic stage and that they shrink dramatically and change their morphology to that of neuron-like cells through active neurite extension. Furthermore, we observed micronuclei movement and transient cell nuclei lobulation concurrent to in vitro neurogenesis from hBMSCs and hDPSCs. Our results demonstrate that the differentiation of hPDLSCs, hBMSCs and hDPSCs towards a neural lineage occurs through a dedifferentiation step followed by differentiation to neural phenotypes, and therefore we definitively confirm that the rapid acquisition of the neural phenotype is via a differentiation trait.
Filiaciones:
Bueno, C:
Inst Neurociencias Alicante UMH CSIC, Campus San Juan, Alicante 03550, Spain
Martinez-Morga, M:
Univ Murcia, Fac Med, Dept Human Anat, Murcia 30800, Spain
Univ Murcia, Fac Med, Inst Biomed Res IMIB, Murcia 30800, Spain
Garcia-Bernal, D:
Univ Murcia, Fac Med, Inst Biomed Res IMIB, Internal Med Dept, Murcia 30800, Spain
Univ Murcia, Fac Med, Inst Biomed Res IMIB, Hematopoiet Transplant & Cellular Therapy Unit, Murcia 30800, Spain
Moraleda, J:
Univ Murcia, Fac Med, Inst Biomed Res IMIB, Internal Med Dept, Murcia 30800, Spain
Univ Murcia, Fac Med, Inst Biomed Res IMIB, Hematopoiet Transplant & Cellular Therapy Unit, Murcia 30800, Spain
:
Inst Neurociencias Alicante UMH CSIC, Campus San Juan, Alicante 03550, Spain
gold, Green Published
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