miR-137 and miR-122, two outer subventricular zone non-coding RNAs, regulate basal progenitor expansion and neuronal differentiation
Por:
Tomasello, U, Klingler, E, Niquille, M, Mule, N, Santinha, A, de Vevey, L, Prados, J, Platt, R, Borrell, V, Jabaudon, D and Dayer, A
Publicada:
15 feb 2022
Resumen:
Cortical expansion in primate brains relies on enlargement of germinal zones during a prolonged developmental period. Although most mammals have two cortical germinal zones, the ventricular zone (VZ) and sub ventricular zone (SVZ), gyrencephalic species display an additional germinal zone, the outer subventricular zone (oSVZ), which increases the number and diversity of neurons generated during corticogenesis. How the oSVZ emerged during evolution is poorly understood, but recent studies suggest a role for non-coding RNAs, which allow tight genetic program regulation during development. Here, using in vivo functional genetics, single-cell RNA sequencing, live imaging, and electrophysiology to assess progenitor and neuronal properties in mice, we identify two oSVZ-expressed microRNAs (miRNAs), miR-137 and miR-122, which regulate key cellular features of cortical expansion. miR-137 promotes basal progenitor self-replication and superficial layer neuron fate, whereas miR-122 decreases the pace of neuronal differentiation. These findings support a cell-type-specific role of miRNA-mediated gene expression in cortical expansion.
Filiaciones:
Tomasello, U:
Univ Geneva, Dept Basic Neurosci, CH-1205 Geneva, Switzerland
CSIC, Inst Neurociencias, Sant Joan dAlacant 03550, Alacant, Spain
Univ Miguel Hernandez, Sant Joan dAlacant 03550, Alacant, Spain
Klingler, E:
Univ Geneva, Dept Basic Neurosci, CH-1205 Geneva, Switzerland
Niquille, M:
Univ Geneva, Dept Basic Neurosci, CH-1205 Geneva, Switzerland
Geneva Univ Hosp, Dept Psychiat, CH-1205 Geneva, Switzerland
Mule, N:
Univ Geneva, Dept Basic Neurosci, CH-1205 Geneva, Switzerland
de Vevey, L:
Univ Geneva, Dept Basic Neurosci, CH-1205 Geneva, Switzerland
Prados, J:
Geneva Univ Hosp, Dept Psychiat, CH-1205 Geneva, Switzerland
Platt, R:
Swiss Fed Inst Technol, Dept Biosyst Sci & Engn, CH-4058 Basel, Switzerland
:
CSIC, Inst Neurociencias, Sant Joan dAlacant 03550, Alacant, Spain
Univ Miguel Hernandez, Sant Joan dAlacant 03550, Alacant, Spain
Jabaudon, D:
Univ Geneva, Dept Basic Neurosci, CH-1205 Geneva, Switzerland
Geneva Univ Hosp, Neurol Clin, CH-1205 Geneva, Switzerland
Dayer, A:
Univ Geneva, Dept Basic Neurosci, CH-1205 Geneva, Switzerland
Geneva Univ Hosp, Dept Psychiat, CH-1205 Geneva, Switzerland
Green Published, gold
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