Mapping microglia and astrocyte activation in vivo using diffusion MRI
Por:
Garcia-Hernandez, R, Cerda, A, Carpena, A, Drakesmith, M, Koller, K, Jones, D, Canals, S and De Santis, S
Publicada:
27 may 2022
Resumen:
While glia are increasingly implicated in the pathophysiology of psychiatric and neurodegenerative disorders, available methods for imaging these cells in vivo involve either invasive procedures or positron emission tomography radiotracers, which afford low resolution and specificity. Here, we present a noninvasive diffusion-weighted magnetic resonance imaging (MRI) method to image changes in glia morphology. Using rat models of neuroinflammation, degeneration, and demyelination, we demonstrate that diffusion-weighted MRI carries a fingerprint of microglia and astrocyte activation and that specific signatures from each population can be quantified noninvasively. The method is sensitive to changes in glia morphology and proliferation, providing a quantitative account of neuroinflammation, regardless of the existence of a concomitant neuronal loss or demyelinating injury. We prove the translational value of the approach showing significant associations between MRI and histological microglia markers in humans. This framework holds the potential to transform basic and clinical research by clarifying the role of inflammation in health and disease.
Filiaciones:
Garcia-Hernandez, R:
CSIC UMH, Inst Neurociencias, Alicante, Spain
Cerda, A:
CSIC UMH, Inst Neurociencias, Alicante, Spain
Carpena, A:
CSIC UMH, Inst Neurociencias, Alicante, Spain
Drakesmith, M:
Cardiff Univ, Sch Psychol, CUBRIC, Cardiff, Wales
Publ Hlth Wales, Communicable Dis Surveillance Ctr, 2 Capital Quarter,Tyndall St, Cardiff CF10 4BZ, Wales
Koller, K:
Cardiff Univ, Sch Psychol, CUBRIC, Cardiff, Wales
Jones, D:
Cardiff Univ, Sch Psychol, CUBRIC, Cardiff, Wales
:
CSIC UMH, Inst Neurociencias, Alicante, Spain
De Santis, S:
CSIC UMH, Inst Neurociencias, Alicante, Spain
Cardiff Univ, Sch Psychol, CUBRIC, Cardiff, Wales
Green Published, gold
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